Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEIEASDLVETVANIRRYQMFGINLSMPYKEQVIPYLDKLSDEARLIGAVNTVVNENGNLIGYNTDGKGFFKCLP--SFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRS----VSMEKTRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGM---DGQSSPVPENIVLPETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIWQSLTEKYQ 3TOZ Chain:D ((32-314)) -RITGHTELIGLIATPIRHSLSPTMHNEAFAKLGLDYVYLAFEVGDKELKDVVQGFRAMNLRGWNVSMPNKTNIHKYLDKLSPAAELVGAVNTVVNDDGVLTGHITDGTGYMRALKEAGHDIIGKKMTICGAGGAATAICIQAALDGVKEISIFNRKDDFYANAEKT---VEKINSKTDCKAQLFDIEDHEQLRKEIAESVIFTNATGVGMKPFEGETLLPSADMLRPE-LIVSDVVYKPTKTRLLEIAEEQGCQTLNGLGMMLWQGAKAFEIWTHKEMPVDYIKEIL-----

General information: TITO was launched using: RESULT: Template: 3TOZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1552 -50013 -32.22 -182.53 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain D : 0.82 3D Compatibility (PKB) : -32.22 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.537

(partial model without unconserved sides chains): PDB file : Tito_3TOZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TOZ-query.scw PDB file : Tito_Scwrl_3TOZ.pdb: