Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MCNVQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSIIIALKISQIFNETVESVFRLEEDE 2B5A Chain:D ((15-76)) ---------LKKIRTQKGVSQEELADLAGLHRTYISEVERGDRNISLINIHKICAALDIPASTFFRKMEEE

General information: TITO was launched using: RESULT: Template: 2B5A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 188 -28699 -152.65 -462.88 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain D : 0.76 3D Compatibility (PKB) : -152.65 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.620

(partial model without unconserved sides chains): PDB file : Tito_2B5A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2B5A-query.scw PDB file : Tito_Scwrl_2B5A.pdb: