Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRQGFIKAFANLVGWIFALIMGAKYAVLLAPSMSGLSQDPVVQKIAAFAFIALLIIVLTWIVTAF--LNGLLKSLKLGPLNRLAGGAFGSLKGLLVVLITMQGVGPWVESSPHWKQSKFIQFLLPYAPLATELSK----DAASEAFHQITSGGGVTRTSPKPMDESEETELRPDHSTKDPFY 5B64 Chain:B ((8-123)) -------------------------------------NNSLMRLREQIVKANLLVREASYIAEELDKRTEYKVTLQIPTSSLDANRKRGSLLSEPAIQVRRKGKG-----KQIWSLEKLENRLLDMRDLYQEWKECEEDSPVSRSYFKRADPFYDEQE------------------------

General information: TITO was launched using: RESULT: Template: 5B64.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 334 -6116 -18.31 -55.60 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.57 3D Compatibility (PKB) : -18.31 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.228

(partial model without unconserved sides chains): PDB file : Tito_5B64.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5B64-query.scw PDB file : Tito_Scwrl_5B64.pdb: