Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKVVVFSQIDEEILSRLQQDYHVVVLNPKLGDINEQIRQEVVDADGMIGA-GRLLNESNLSPAQKLKIISSVTVGYDNYDVGYLNQKKIWLANTPHVLTETTADLAFTLLLSAARKVPFLDHWTKQGEWKR---TVGPQQF-GLDVFGKTLGIIGLGNIGAAIARRGFYGFNMNIVYHNRREKPELAEPLKAQYLGLEELLQQSDFVVTAVGLNAESKALMGKAQFELMQKHAIFINIARGSVVDEQALIEALQNEVIFAAGLDVYEKEPLQESALFNLPNVVTLPHVGSATAETRKKMANLAYKNLVEALEDKTPRYLVNPNFV 2DBR Chain:D ((4-322)) --KVFITREIPEVGIKMLEDEFEVEVWGDEKEIPREILLKKVKEVDALVTMLSERIDKEVFENAPKLRIVANYAVGYDNIDIEEATKRGIYVTNTPDVLTDATADLAFALLLATARHVVKGDRFVRSGEWKKRGVAWHPKWFLGYDVYGKTIGIIGLGRIGQAIAKRA-KGFNMRILYYSRTRKEEVERELNAEFKPLEDLLRESDFVVLAVPLTRETYHLINEERLKLMKKTAILINIARGKVVDTNALVKALKEGWIAGAGLDVFEEEPYYNEELFKLDNVVLTPHIGSASFGAREGMAELVAKNLIAFKRGEIPPTLVN----

General information: TITO was launched using: RESULT: Template: 2DBR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1648 1097 0.67 3.49 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain D : 0.83 3D Compatibility (PKB) : 0.67 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.543

(partial model without unconserved sides chains): PDB file : Tito_2DBR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2DBR-query.scw PDB file : Tito_Scwrl_2DBR.pdb: