Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIVLGLETSCDETGLALYDSELGLRGQVLYSQIKL--HAEYGGVVPELASRDHVRKLIPLMNQLLEQSGVKKQEIDAVAYTRGPGLMGALMTGALFGRTLAFSLNKPAIGVHHMEGHMLAPLLSS--QPPEFPFVALLVSGGHTQLMVVHGIGQYELLGESIDDAAGEAFDKVAKMMNLPYPGGPNIAK---------LALSGDPLAFEFPRPMLHQG----LDFSFSGLKTAVSVQLKKLNG-------ENRDADIAASFQEAIVDTLVKKSVKALKQ-----TGLKRLVIAGGVSANLRLREQLETSLARIKA-QVYYAEPALCTDNGAMIAFAGYQRLKAGQ-HDGLAVTTTPRWPMTELTIPE 3WUH Chain:B ((11-372)) --VLAIETSCDDTCVSVLDRFSKSAAPNVLANLKDTL----------KAHIHHQARIGPLTERALIESNAR-EGIDLICVTRGPGMPGSLSGGLDFAKGLAVAWNKPLIGVHHMLGHLLIPRMGTNGKVPQFPFVSLLVSGGHTTFVLSRAIDDHEILCDTIDIAVGDSLDKCGRELGFK---GTMIAREMEKFINQDINDQDFALKLEMPS------KRNMLSFSFSAFITALRTNLTKLGKTEIQELPEREIRSIAYQVQESVFDHIINKLKHVLKSQPEKFKNVREFVCSGGVSSNQRLRTKLETEL-----FNFYYPPMDLCSDNSIMIGWAGIEIWESLRLVSDLDICPIRQWPLNDL----

General information: TITO was launched using: RESULT: Template: 3WUH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1530 -20802 -13.60 -68.20 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.78 3D Compatibility (PKB) : -13.60 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.553

(partial model without unconserved sides chains): PDB file : Tito_3WUH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3WUH-query.scw PDB file : Tito_Scwrl_3WUH.pdb: