Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MEFVFNGFYTLISAVIVLLLGRFLVNRIDFLERYNIPEPVAGGLVAAVVSLLVHTFWGYSIVFSSELQTSFMLVFFASIGLSANFMKLKEGGTALVIFLICVASFIVVQNAVGMSLATLLGLDPLIGLIAGSITLTGGHGTAGAWGEILESQHGIQGALALGMASATFGLIMCVIGGPLAKLLINRYSLAQAKTNAEIQQRDTHVEQNSDDLAPFENPHQVRLITADNAITTLGMFAACLAFAEFMTGFSKGTWFELPTFVWALGGGVILRNILESVLKVDIFDRAIDVFGNASLSLYLAMALLSLKLWQLADLAGPLVVILVAQTLTMALYAAFVTFRVMGKNYDAAVLAASHCGFGMGATPTAVANMQAITNMYGPSHKAFLIVPLCGAFFVDLINATVIQLMLKFIA------- 4BWZ Chain:A ((3-386)) GAEHLLEIFYLLLAAQVCAFIFKRL------NQPVVIGEVLAGVLVGPALLGLVHE--GEILEFLAELGAVFLLFMVGLETRLKDILAV--GKEAFLVAVLGVALPFLGGYLYGLEI----GFETLPALFLGTALVAT---SVGITARVLQEL----GVLSRPYSRIILGAAVIDDVLGLIVLACVNGVAETGQVEVGAITRLIVLSVVFVGLAVFLSTLIARLPLERLPVGSPLGFALALGVGMAALAASIG----LAPIVGAFLGGMLLSEVREKY------RLEEPIFAIESFLAPIFFAMVGVRL-ELSALASPVVLVAGTVVTVIAILGKVLGGFLGA--LTQGVRSALTVGCGMAPRGEVGLIVAALGLKAGAVNEEEYAIVLFMVVFTTLFAPFALKPLIAWTERERAAKE

General information: TITO was launched using: RESULT: Template: 4BWZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2129 -293742 -137.97 -781.23 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -137.97 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.339

(partial model without unconserved sides chains): PDB file : Tito_4BWZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4BWZ-query.scw PDB file : Tito_Scwrl_4BWZ.pdb: