Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNTFVDAARSFRTQFDLNFSEKIIVDFFAGGGGASTGLEMGLNRPVYVAVNHNPKAISMHEANHPHAKHYIQDVFAVDPIDI----CDGHQVGWFHASPDCTHHSQAAGGQPRKKEIRDLSWVVLKFAGKVKPDVISLENVKQILGWGPLIAKRDKATGRVITLDKININGKKVNRIAEPGERVPRHNQFLVPNPKKKGKTWKHFVRSLEQLGYEVE-WQKNIIAADFGAPTKRERLFLIARCDGQPIVWPEKYFSKKPKGNLKKWRSTVECVDFSDLGNSIFDRPQGPLADATLKRIAKGIQ-KYVIETKEPFFVNSSTPFIGRDFRTSFGHDIREPLATTTAGYGGHSSLISPILVPFITEFANASQQRNWSIDEPLSTICAQVKGGHHGLVTAKLSKDNYKGALRVAAFL-INYYGNGDARSITEPMDTITTKDRLVLVTVWIKGEPWAIVDICIRMLKPRELFRAQGVPDSYVIEYGSDGKPLSKKDQVFMVGNSVSPYPMAAIARANNPFITQQIKGAA 3G7U Chain:A ((2-369)) --------------------SLNVIDLFSGVGGLSLGAARA-GFDVKMAVEIDQHAINTHAINFPRSLHVQEDVSLLNAEIIKGFFKNDMPIDGIIGGPPCQGFS--------DDSRNQLYMHFYRLVSELQPLFFLAENVPGIMQEKY--------------------------------------------------SGIRNKAFNLVSGDYDILDPIK-VKASDYGAPTIRTRYFFIGVKKSLKLDISDEVFMP----KMIDPVTVKDALYGLPD-IIDAN---WQSDSESWRTIKKDRKGGFYEK-------------L----------WGQIPRNVGD----TE--SI-AKLKN----------NIISGCTGT-----------LHS----KIVQERYA--S--LSFGETDKISRSTRLDPNGFCPTL----------------VRPIHPYHPRVITPREAARLQGFPDWFRFH------VT-KWHSFRQIGNSVSPIVAEYILKGLYNLLNE------

General information: TITO was launched using: RESULT: Template: 3G7U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1960 -38952 -19.87 -114.23 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -19.87 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.246

(partial model without unconserved sides chains): PDB file : Tito_3G7U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3G7U-query.scw PDB file : Tito_Scwrl_3G7U.pdb: