Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQDDISGWTEWNAHFEGIEEISSPSELHGLLTGI-V-CVT-EAPTLEEWTQ-ILTT-LNVPELNEEALALLTDEAEDVAHALSEDELDYLPMLPDDEHLLQDRVQALSDWCAGVVLGFGLASGHVRTDERELIEHLQDVAAVEFEDSDNDEEGESSYEELYEFVRLIPVSLSIGRKKVTVAESSLLKNFYAKSKTSTVGTADQNIVEMFTPHRPS 2Q82 Chain:A ((14-128)) ---------------------ELQNRLAQYETSLMVMSHNGDVPVITGFNVMRVTTMLDALKVP--AVAVLGDDAQDLAYVFGARPLAVGVNII--------RVVDVPGQQPSALVDAELGALHEVSM----VRVLNDIADEQLVKAN-------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2Q82.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 486 -2602 -5.35 -24.09 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -5.35 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.334

(partial model without unconserved sides chains): PDB file : Tito_2Q82.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Q82-query.scw PDB file : Tito_Scwrl_2Q82.pdb: