Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKQYMTHRCLIAPPEMADDFFANTVIYLARHDEEGAQGIIINRPAGIQIKELLNDLDIDA---DNVNPHEVLQGGPLRPEAGFVLHTGQPTWHSSIAVGENVCITTSKDILDAIAHNEGVGRYQIALGYASWGKNQLEDEIARGDWLICDADMDLIFNLPYDDRWDAAYKKIGVDRTWLASEIGHA 2MUI Chain:A ((5-189)) -SPTYLKHHFLIAMPHMADPNFAQTVTYLVEHNEQGAMGLVINRPSGLNLAEVLEQLKPDALPPARCQHIDIYNGGPVQTDRGFVLHPSGLSYQ-STLELGELAMSTSQDVLFAIAAGTGPEKSLISLGYAGWEAGQLEAELSDNAWLTCPADPAILFDLPPEERLSAAAARLGVNLSLLTAQAGHA

General information: TITO was launched using: RESULT: Template: 2MUI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 870 -33370 -38.36 -183.35 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : -38.36 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.459

(partial model without unconserved sides chains): PDB file : Tito_2MUI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2MUI-query.scw PDB file : Tito_Scwrl_2MUI.pdb: