Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQIYLARNNQQAGPYTLEQLNQMLASQQVLLTDLAWHEGMTEWKALGELTQGKLVYQPTGYSAFSA-NTNTPYNETIQHIRV-ETKTHELASISSRALAK-IIDLLLWLPIAAIPSFFFN-EAQYKQLFELQKQMQSAEVASTKAAELQQQLFTLIPIEAWHTML-------LYVVIMLAIQAFLLTKFGQSIGKKIVGIRIVDAENNGKVNLTRIFLLRSVVFIILNLLFMPISTIIDYAFALGQKRQALHDKIARTKVIK 5KXF Chain:A ((30-156)) -------------------------------------------------------------HIWRGMIAKGGTPVCCARCVPMETKLPEVVNCSARTDLNMLAKHYAVAIGCEIVFFVPDREEDFASYTEFLRYLSSKDRAGVAKLDDGTTLFLVPPSDFLTDVLQVTRQERLYGVVLKLPPPA------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5KXF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 467 -19717 -42.22 -176.04 target 2D structure prediction score : 0.31 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : -42.22 2D Compatibility (Sec. Struct. Predict.) : 0.31 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.197

(partial model without unconserved sides chains): PDB file : Tito_5KXF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5KXF-query.scw PDB file : Tito_Scwrl_5KXF.pdb: