Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNSFMQQSLKMKKILMMSMCGALLLTGCNKTSQQDQTVSKEQKSTAQKTTEQRDSSCLQIMVAMHTINQNSKIEDLNQINEKLKTCVPSLKSEEQLKLIEASTAMYQRFLKQDYTDKTARAFEAFGYAVLEQKQDLKKVIQSQKKLFAQLSPRDQYLLQHEGQAYIELLYQGEGMFTYRRQPNYLIDVFSKALPADQKEFLSRMAKDNQDIFYNDGALAVSWKELTERALFWEKFIQKYP--KSYFINDAKLL--FNEYRYFIFFGLDNTPVSNEYAPNTWFDPDALQQIRFLSTQSQSSLAKPAQKFLKFIATPVDERNKQFKIDLTDENGQ-----KKSTYQIVHEQLEQLLKFDSPWNTEIYRDCHIDAVCIDTN 1FT1 Chain:A ((55-369)) -----FLSLDSPTYVLYRDRAEW-------ADIDPVPQNDGPSPVVQIIYSEKFRDVYDYFRAVLQRDERS----------------------------ERAFKLTRDAIELNAAN--YTVWHFRRVLLRSLQKDLQEEMNYIIAIIEE-QPKNYQVWHHRRVLVEWL-----------KDPSQELEFIADILNQD---------AKNYHAWQHRQWVIQEFRLWDNELQYVDQLLKEDVRNNSVWNQRHFVISNTTGYSDRAVLEREVQYTLEMIKLVPHNESAWNYLKGILQDRGLSRYPNLLNQLLDLQPSHSSPYLIAFLVDIYEDMLENQCDNKEDILNKALELCEILAKEKDTIRKEYWRYIGRSLQSKHSR

General information: TITO was launched using: RESULT: Template: 1FT1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1183 -24218 -20.47 -79.14 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -20.47 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.242

(partial model without unconserved sides chains): PDB file : Tito_1FT1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FT1-query.scw PDB file : Tito_Scwrl_1FT1.pdb: