Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKYCQFFSVLALGL-SAASCAVT-SGLQTYDIPSEG-VYKTDLGTTVNVVKISQETLPAIQPAQIDYQRDYASLFKNQQTIYRLSPGDVLSIQLWAYPEIAPPVNNISNEQSVQAYGYPIDQTGYIQFPLVGRYKAAGKTLAQVNRELHSQLARFLKNP-----DVVVRVVSYEGQRFSVQGSVTKGGQFYLSDQPVSIYTALGMAGGVTTTGDNTYIQLIRNGRTYNLNTIDLEKAGYSLHKLLVQPND---------TIYVSTRENQKIYVMGESGKNQALPMRDQGMSLTDALGESLGINPLSGSASRIYVVRTNPNDRTTE------IYHLNLMSIGDFGLANQFRLRSNDVVYVDATGLTRWQRIVN--------QIIPFSNALYNIDRLGQ------------ 4MSX Chain:A ((63-482)) YAYLETVVREKLDFDSEKICCITLSPLNVYCCLVCGHYYQGRHEKSPAFIHSIDENHHVFLNLT---SLKFYMLPQNVQILHDGEVQLLNSIKFAAYPTYCPKDLEDFPRQCFDLSN-RTYLNGFIGFTNAATYDYAHSVLLLISH-MVPVRDHFLLNHFDNQGEFIKRLSICVKKIWS-----PKLFKHHLSVDDFVSYLKVREGLNLNPIDPRLFLLWLFNKICSSSNDLKSILNHSCKGKVKIAKSESVTGKVIVKPFWVLTLDLPEFSPFEDGNSVDDLPQINITKLLTKFTKTVFELTRLP----QFLIFHFNRFDRNSDHPVKNRNQTLVEFSSELEILHVKYRLKANVVHVVIGDEKSHWITQLYDNKSEKWIEIDGINTTEREAELLFLKETFIQVWEKQE

General information: TITO was launched using: RESULT: Template: 4MSX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1551 -12319 -7.94 -35.00 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -7.94 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.045

(partial model without unconserved sides chains): PDB file : Tito_4MSX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MSX-query.scw PDB file : Tito_Scwrl_4MSX.pdb: