Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNAPVKTENHLSVQQESPIDKTLSMIERIALDPSSNVAKLEKMIELQERVMKTQAKQAYDNAMVLAQSEMPPIDKFKKGHNSNYAPLDHIMSIVFPVLKRNNLFVRWTSDPKENGSLCVTCICSHVGGHSETSSMDVKEDRGGSKSDIQGMGSAFTYAKRYTLSALLGLVLTDDTDGARINLKVTDAQATMLRNKLKFFKPEALEAFKAKIGCEVEELPRGDFDYWCSYIDNQINKLMANQGEKNANP 2W0C Chain:P ((1-65)) --------------MNTSVPTSVPTNQSVWGNVSTG---LDALISGWARVEQIKAAKASTGQGRVEQAMTPELDNGAAVVVE----------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2W0C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain P - contact count / total energy / energy per contact / energy per residue : 22 1959 89.02 30.13 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain P : 0.56 3D Compatibility (PKB) : 89.02 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.290

(partial model without unconserved sides chains): PDB file : Tito_2W0C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2W0C-query.scw PDB file : Tito_Scwrl_2W0C.pdb: