Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----MGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQ-----TTHAAIAVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPN------------------RVNVLLVATRTENVETRVEVLELADLNPKLADVESYAVERTFSVFADSLPIGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEEASRAKKDR-----SLPDDYEIEV---------------LDPFLDAVVQQAARSLQFFFSSSQFNEIDH--ILLAGGNANIPGLAKLLQQKLGYRVTIANPFLQMGFSPQVDVQKIENDASSLMVACGLALRSFD 1E4F Chain:T ((7-390)) TVFYTSIDIGSRYIKGLVLGKRDQEWEALAFSSVKS--RGLDEGEIKDAIAFKESVNTLLKELEEQLQKSLRSDFVISFSSVSFEREDTVIERDFGEEKRSITLDILSEMQSEALEKLKENGKTPLHIFSKRYLLDDERIVFNPLDMKASKIAIEYTSIVVPLKVYEMFYNFLQDTVKSPFQLKSSLVSTAEGV-LTTPEKDRG---VVVVNLGYNFTGLIAYKNGVPIKISYVPVGMKHVIKDVSAVLDTSFEESERLIITHGNAVYNDLKEEEIQYRGLDGNTIKTTTAKKLSVIIHARLREIMSKSKKFFREVEAKIGIPGGVVLTGGGAKIPRINELATEVFKSPVRTGCYAN-SDRPSIINADEVAND-PSFAAAFGNVFA---

General information: TITO was launched using: RESULT: Template: 1E4F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain T - contact count / total energy / energy per contact / energy per residue : 1657 55483 33.48 168.64 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain T : 0.66 3D Compatibility (PKB) : 33.48 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.386

(partial model without unconserved sides chains): PDB file : Tito_1E4F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1E4F-query.scw PDB file : Tito_Scwrl_1E4F.pdb: