Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MWQQLITWLKQLSFLVKKEFLTIFSDPANRAILFVPALMQALL---SGYAATYDVNHVDYAILDQSNGQISHE----LIS-KLDGSGIFKRVATLEYTEQIKQVTDNRQALLIIAIPNDFESKLNNNQSAPIQV---IVDGRNS------STAMVAGSYLNK----------IIGQFNQQKFNSAL-----PISLETRTWYNPNQESRWSLMPALIAA--LSMMQTLLLSALSVAREREQGTFDQLLVTPYTPLQIMIGKALPPIFVGLMQSTIILLIILFWFKIPMNGSIGLLYFG--LFSFNVAV-----------VGVGLS-----------ISALSLNMQQAMLFTFL-----LIMPLMLLSGLLTPVENMPKA-LQVATYANPLRFGINLVQRVYLEG-ASFAQVKLNFLPMIVLGIVTLPLAAWLFRNRLS 5L35 Chain:A ((1-422)) ----PNNLDSNVSQIVLKKFLPGFMSDLVLAKTVDRQLLAGEINSSTGDSVSFKRPH-QFSSLRTPTGDISGQNKNNLISGKATGRVGNYITVAVEYQQLEEAIKLNQLEEILAPVRQRIVTDLETELAHFMMNNGALSLGSPNTPITKWSDVAQTASFLKDLGVNEGENYAVMDPWSAQRLADAQTGLHASDQLVRTAWENAQIPTNFGGIRALMSNGLASRTQGAFGGTLTV---KTQPTVTYNAVKDSYQFTVTLTGATASVTGFLKAGDQVKFTNTYWLQQQTKQA---LYNGATPISFTATVTADANSDSGGDVTVTLSGVPIYDTTNPQYNSVSRQVEAGDAVSVVGTASQTMKPNLFYNKFFCGLGSIPLPKLHSIDSAVATYEGFSIRVHKYADGDANVQKMRFDLLPAYVC--FNPHMGGQFFGNP--

General information: TITO was launched using: RESULT: Template: 5L35.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1392 -65179 -46.82 -182.57 target 2D structure prediction score : 0.23 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -46.82 2D Compatibility (Sec. Struct. Predict.) : 0.23 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.044

(partial model without unconserved sides chains): PDB file : Tito_5L35.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5L35-query.scw PDB file : Tito_Scwrl_5L35.pdb: