Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTPTQNVPEDRIQIGQLRSAYGLNGWLWVYSNTEPMSNMFDYLPWYIE--TKAGWQIVD----VKRWKPHGKGLVVALKGVSDRTGAES---------------LVGANIWIAKSQLPKADVDEYYWSDLKGLTVLGLDDDEQEVNL--GQIHELFETGANDVMVVRATPDSIDSEERMIPWHKDVVQRVDLEAGRIYVNWGVDY 4TSD Chain:A ((2-180)) -----------AIFGELSSL------GHLFKKTQELEILHEYLKEVMQKGSKANQRVLNLATNTEFQVPLGHGIFSIEQSYCLEHAKESEKGFFESHKKYVDFQLIVKGVEGAKAVGINQAVIKNPYDEKRDLIVYEPVSEASFLRLHAGMLAIFFE---NDAHALRFYGESFEKYR------EEPIFKAVVKAPKGLIKLKLAA

General information: TITO was launched using: RESULT: Template: 4TSD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 698 5396 7.73 34.59 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 7.73 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.228

(partial model without unconserved sides chains): PDB file : Tito_4TSD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4TSD-query.scw PDB file : Tito_Scwrl_4TSD.pdb: