Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------MELNLK------EDWSKKMK-TALNRTPASFPVRRMDYNFEDTPRYWCNHEPSLTHYFTGLSTLFPEGESYFVRSVRALRAKAKENEILDREIGAFIGQEAMHSKEHHAFHVSAQQYGLNPESLE---KATGIVLKAIEKVFSKKWNLLVTVGLEHYTAVL-VVSMMQSVNELMTENTIRNLWLWHSVEETEHKAVAYDLYQHLYGNGLDAYIPRVGIFTFSLVLITAFSTIYHIVLMKRDKQLTNFKTWRNFFKFASKQYKVLIPKFLEYYRFDFHPNQ-TDEKALVAATKVKLGINDRSLLLS 4RHJ Chain:A ((19-318)) SHMFSKFLMNVKGVTPRGSDWANRLGPVALFGYGAGMPRRAPLLDFFLQSPRDCDHYAELTIHDKG-PIECPPETVMFMPVLNC-------GQMLDEAAGTETPTSDEWYLGSLEASTELLEKGYVPVSVGGDGSATLSMVEAYKRLFPSD-----DIVIVHFSARPSVSDPRSPLRVLLDKGLLKGVVSVGNRQVSSEDRKVRKLHKMFYMDMRD---IRNDYPVFISIDASVLDPAFAPAV---DSPVAGGLSTRDLLHIMNG---IRGPKVVGIDVYGYNPDLDVYRKDNVGLTAIALSKIIKEGILK

General information: TITO was launched using: RESULT: Template: 4RHJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1448 13683 9.45 50.49 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 9.45 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.203

(partial model without unconserved sides chains): PDB file : Tito_4RHJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RHJ-query.scw PDB file : Tito_Scwrl_4RHJ.pdb: