Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPDENNAMIRILIFISALLFFIRFGNAEDIFNGTILFKNNDWHFVRCSITQDDYLIEAPQEIFT--QFKE----LQQQQKNYWVSVLAEVNEQQNGNLILKIEKIDEVHL---DETCHLLEALKNFENRE 3MXN Chain:B ((72-147)) --------------------------------------------------RGEARLRDPSGDFSVRGLERVPRGRPCLVPGKYVMVMGVV-QACSPEPCLQAVKMTDLSDNPIHESMWELEVEDLHR---

General information: TITO was launched using: RESULT: Template: 3MXN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 244 7510 30.78 112.08 target 2D structure prediction score : 0.30 Monomeric hydrophicity matching model chain B : 0.56 3D Compatibility (PKB) : 30.78 2D Compatibility (Sec. Struct. Predict.) : 0.30 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.367

(partial model without unconserved sides chains): PDB file : Tito_3MXN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3MXN-query.scw PDB file : Tito_Scwrl_3MXN.pdb: