Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTVNAPEFVRHPKLIAWVEEIANLTKPAKIEWCDGSEEEYQRLIDLMIANGTMQKLNQEKHPGSYLANSDPSDVARVEDRTYICSQNKEDA--------GATNNWEDPAVMREKLNGLFEGSMKGRTMYVVPFSMGPLGSHIAHIGIELTDSPYVAVSMRKMARMGKAVYDVLGTDGEFVPCVHTVGAPLAEGQKDVA-WPCNPE-KYIVHYPETREIWSFGSGYGGNALLGKKCLALRIASVMGREQGWLAEHMLILGVTNPQGEKHYIAAAFPSACGKTNFAMLIPPAGYEGWKIETVGDDIAWIKPGEDGRLYAINPEAGFFGVAPGTNTKTNPNCMATLHKDVIYTNVAVTDDGQVWWEGLSKEVP--ANLTNWKGQ---PHVNGEKAAHPNARFTVAAGQCPSIDADWENPAGVPISAFIFGGRRADTVPLVSEAFDWVDGVYKAATMGSETTAAAVGQQGIVRRDPFAMLPFAGYNMADYFDHWLNLGAKVSEKAEASGNKLPKIFNVNWFRRDAEGNFVWPGFGQNMRVLEWIIDRCEGRANAVETPIGFVPTYEDLNWEGT-EFTKEQFDLITNQDKDQWVTEIESHTELFNKLGERLPKALKERQAALLEAVKTGF 4YWD Chain:A ((30-619)) -----------------FVEGNAQLCQPEYIHICDGSEEEYGRLLAHMQEEGVIRKL--KKYDNCWLALTDPRDVARIESKTVIITQEQRDTVPIPKSGQSQLGRWMSEEDFEKAFNARFPGCMKGRTMYVIPFSMGPLGSPLAKIGIELTDSPYVVASMRIMTRMGTSVLEALG-DGEFIKCLHSVGCPLPLKKPLVNNWACNPELTLIAHLPDRREIISFGSGYGGNSLLGKKCFALRIASRLAKEEGWLAEHMLILGITNPEGKKKYLAAAFPSACGKTNLAMMNP--TLPGWKVECVGDDIAWMKFDAQGNLRAINPENGFFGVAPGTSVKTNPNAIKTIQKNTIFTNVAETSDGGVYWEGIDEPLAPGVTITSWKNKEWRP----EPCAHPNSRFCTPASQCPIIDPAWESPEGVPIEGIIFGGRRPAGVPLVYEALSWQHGVFVGAAMRSEAT------GKVIMHDPFAMRPFFGYNFGKYLAHWLSM-------AHRPAAKLPKIFHVNWFRKDKNGKFLWPGFGENSRVLEWMFGRIEGEDSAKLTPIGYVPKEDALNLKGLGDVNVEELFGISKEFWEKEVEEIDKYLE--DQVNADLPYEIERELRALKQRI----

General information: TITO was launched using: RESULT: Template: 4YWD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3404 20159 5.92 35.68 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : 5.92 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.612

(partial model without unconserved sides chains): PDB file : Tito_4YWD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4YWD-query.scw PDB file : Tito_Scwrl_4YWD.pdb: