Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSTQPMIEKLIEAHLDFLDEQFAQTQVIQQEFEQFYHWLGSRQLQHLWTFEQVQQLIQKQILDTPASDFLIEQIAEHIRFALIHPTNDTATVEDVIPVLTIDRIAQYVASKEEHRKKLIKTIVNNPAFSALLTQLIQQTMHDYLDESMTKRVPGVGRFMKMGKSVLETVTDSNLDDTINHYLQKNILKLSQMSKRVLNQHFDNDKLYHFQANVWHKVKTSPLSVLKNYIEVQDLTRTVGLGHEIWDHIRQTDYLKQQVHDGIYTWYVRNQERNFDLLLRDLNIDENLVKHELTELLVPVLQQLVTTGHLRRRARVYLEKFYYSEKALEILNNKGA 2C7L Chain:B ((84-142)) -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RDLGFILR---YVTYSVLAGDTSVMDDRCLNGLRETYQALGTPGDAVASGIKKMKEAALKIA--------------------------------

General information: TITO was launched using: RESULT: Template: 2C7L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 106 -16418 -154.89 -278.27 target 2D structure prediction score : 0.83 Monomeric hydrophicity matching model chain B : 0.54 3D Compatibility (PKB) : -154.89 2D Compatibility (Sec. Struct. Predict.) : 0.83 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.369

(partial model without unconserved sides chains): PDB file : Tito_2C7L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2C7L-query.scw PDB file : Tito_Scwrl_2C7L.pdb: