Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTRPDSKSMNYQLLKTFSRQPIQFGRFLARLLAGLVNTLKITRTSKSIELNLRIALPYLTPQQRIAITEKAVRNELTSYFEFLSIWGSSNSKNISRIHRIEGEHFFHEALAAKKGVVLIVPHFGTWEVMNAWCAQFTSMTILYKPVKNADADRFVREARSREQANLVPTDESGVRQIFKALKQGETTVILPDHTPNVGGDMVNYFGVPLASSNLSAKLIQKTKAKALFLYAIRNENDGFTIHIEPMDEKIYEGTADDGTYVIHQAIEQLIYQYPEHYHWSYKRFKANPALDNIYNIDPTEALKIVDRLKAEALKTSTQPEPIQTSVM 1E6Y Chain:C ((220-247)) ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NVAFRDDAEVVEWVHRIFDQRTKFGFQPK-------

General information: TITO was launched using: RESULT: Template: 1E6Y.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 13 860 66.15 30.71 target 2D structure prediction score : 0.93 Monomeric hydrophicity matching model chain C : 0.52 3D Compatibility (PKB) : 66.15 2D Compatibility (Sec. Struct. Predict.) : 0.93 1D Compatibility (Hydrophobicity) : 0.52 QMean score : 0.646

(partial model without unconserved sides chains): PDB file : Tito_1E6Y.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1E6Y-query.scw PDB file : Tito_Scwrl_1E6Y.pdb: