Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNAPVKTENHLSVQQESPIDKTLSMIERIALDPSSDVAKLEKMIELQERVMKTQAKQAYDNAMVLAQSEMPPIDKFKKGHNSNYAPLDHIMSIVFPVLKRNNLFVRWTSDPKENGSLCVTCICSHVGGHSETSSMDV--KEDRGGSKSDIQGMGSAFTYAKRYTLSALLGLVLTDDTDGARINLKVTDAQATMLRNKLKFFKPEALEAFKAKIGCEVEELPRGDFDYWCSYIDNQINKLMANQGEKNANP 1YAR Chain:O ((10-237)) ----KRAALIQNLRDSYTETSSFAVIEEWAAGTLQEIEGIAKAAAEAHGVIRNS-TYGRAQAEKSPEQLLGVLQRYQDLCHNVYCQAETIRTVIAIRIPEHK--------EEDNLGVAVQHAVLKIIDELEIKTLGSGEKSGSGGAPTPIGMYALREYLSARSTVEDKLLGG-GSQSPSLLLELRQIDADFMLKVELATTHLSTMVRAVINAYLLNWKKLIQ------------------PRTGSDHMVS

General information: TITO was launched using: RESULT: Template: 1YAR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain O - contact count / total energy / energy per contact / energy per residue : 768 -16137 -21.01 -74.71 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain O : 0.66 3D Compatibility (PKB) : -21.01 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.239

(partial model without unconserved sides chains): PDB file : Tito_1YAR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1YAR-query.scw PDB file : Tito_Scwrl_1YAR.pdb: