Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------MKKITALLFALC-ATVTHAELQTL-------DNDALQAINGQAGADLSLMLN---------LNQTSNGIFDNGSGG----------VCENVEFCHIGLAINKRFVQADTTKPS----GWAENSDSGNKLWLVFKGVQGTLNIQKMGLDGADLKYLDKSN---------TEIIKLAIQLSF---SAAQPILIRNF-GFNALSIEQDNFTSSTTTQGSSANMYDYAYFKKPTYAATANSANQQVA---TATPSAYDYGRETGFMGLMMNGNMAIQGKVMVFSCDGSHPRC 5JUF Chain:A ((1-299)) MNLKDSIGLRIKTERERQQMSREVLCLDGAELTVRQLIRIEKGESLPSLDRLSYIAKRLGKSMTELLDQDNITIPDEYYEMKNRLIKFPTYRNPDRIKSKLTLIEEVYEKFFDILPEEELLTLDILENILSFTSWEESPKVEEIYEDLFEQVKRKRKFSTNDLLVIDYYFFHLYGRKQYDKKLFERIIKRVLNQEIWTDDVYNIVLFNDLMAIAALKIFHNSFSDFLTVVDKALAVIEKSQLYSYKPSVFVLKAKYELLHKENKKEAAENYDKAIVFASVLEDSVLEESIKAGKLA-DGL----

General information: TITO was launched using: RESULT: Template: 5JUF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 925 11185 12.09 47.39 target 2D structure prediction score : 0.25 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 12.09 2D Compatibility (Sec. Struct. Predict.) : 0.25 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.182

(partial model without unconserved sides chains): PDB file : Tito_5JUF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5JUF-query.scw PDB file : Tito_Scwrl_5JUF.pdb: