Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPPRIRRSVMRQFTSLQVAILALGSLCFSTAYADSTLVPMSDAELSATRGQALMSMSYIAPNDSANLEKLRDSSSNVGFYKLGLEAELEINANIRKLQLGCGGVNGAGGCDIDFDNVSLSGVADTREGRV---ASDAKLTNPFLEFAIKNPNSASTREVAGIRLSAEAVEG-----LLTIGTENSATPNGINSLSGY--MVVAPQVGEATVDAARITQTGSPACGVYPSPAGCGVNQAITGKARGKIALGAGFNLDFQTKSYDITLTPTQKAQLSLPQSVVSGQRMSSVNLLASAIVNGIDLSGTLAADVDILGGITLNGNLRGTINNLPVTVPLLENLGYIHKINLSGSPLSLSMQGQDIRWPGTVSTAMRGWWLELSNPIDIGRIDPTNSVVIKTDTIRDALTEVSKELTQNPLNCGFLAVNCIGGDFNVGTRDLSNARPALLELQNLQLANQSFAPNCYGSLKFC 5B5Z Chain:A ((15-277)) ------------------------------------------------------------------------------------------NVTLTAVKKAFPDA---------LTNAELVAMVSKRLSQFGYHKYNTLLATSLCSDEVTRPLEQDFGEVYGKHFTMGGLAGFPFGGLTGFGAMAGAIPDGGSCLLIYGSHVGVSWEGKWGTVARRGREKGGACC-----GSAVAAAQAVTQAYQATPLDAQQGYVRDMLRPYAATLSEAEDVMVTLPVSVYDAQQKLVTRILDEGSNH-IDGDG----QIAVVGGIQINTPKEMSDFFVVRRFCIRDSSGNMVENFMPL-----------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5B5Z.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1251 20309 16.23 88.30 target 2D structure prediction score : 0.40 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : 16.23 2D Compatibility (Sec. Struct. Predict.) : 0.40 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.011

(partial model without unconserved sides chains): PDB file : Tito_5B5Z.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5B5Z-query.scw PDB file : Tito_Scwrl_5B5Z.pdb: