Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MINIQNTILGKIVDRKHEDLAARLKQRNLQDVEELAKAATPVRGFANALQ----HKRPGVIAEIKKASPSKGIIRADFNPAEIAQQYEQAGAACLSVLTDVDFFQGADENIAIARNHCTLPALRKDFLVDPYNVVEARALHADCILLIVACLSDQQLEEMSKTAFEHQLDVLVEVHDEEELERALKLSEQCLLGVNNRNLKTFDVDLNTTIRLKKLLPASRLLITESGIATPDDVRMMQEHDIHSFLVGESFMKQPRPDQAFTALFGQPQTV 3TSM Chain:B ((9-268)) -------ILRKIEAYKREEIAAAKARLALDELKARTRDQSAPRGFLKALEAKRAAGQFALIAEIKKASPSKGLIRPDFDPPALAKAYEEGGAACLSVLTDTPSFQGAPEFLTAARQACSLPALRKDFLFDPYQVYEARSWGADCILIIMASVDDDLAKELEDTAFALGMDALIEVHDEAEMERALKLS-SRLLGVNNRNLRSFEVNLAVSERLAKMAPSDRLLVGESGIFTHEDCLRLEKSGIGTFLIGESLMRQHDVAAATRALLTG----

General information: TITO was launched using: RESULT: Template: 3TSM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1313 19776 15.06 77.25 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain B : 0.85 3D Compatibility (PKB) : 15.06 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.457

(partial model without unconserved sides chains): PDB file : Tito_3TSM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TSM-query.scw PDB file : Tito_Scwrl_3TSM.pdb: