Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLNLMLGFASGVMIAASFWS------LLDPAITKAEENGDIAWLVV-SIGFGLGGVFLYMADKTLPHMHFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVSVALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELLLPYALAF--AAGAMIYVVVEEL----------IPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG 4Q0S Chain:A ((12-260)) SARTSITRREYDEWVREAAALGKAL-RYPITEKMVNDSAGIVFGADQYDAFKNGMWSGEPYEAMIIFESLNEPAVDGLPTGAAPYAEYSGLCDKLMIVHPG------------------KFCPPHH------HGRKT--ESYEVVLG------EMEVFY-SPTPSAESGVELLNFSGMPVG---SPWPEGVALPKG-RESSYEKLTSYVRLRAGD--------------PKFVMHRKHLHAFRCPPDSDVPLVVREVSTYSHEPTEAAAGNHAPIPSWLGMHDNDFVSDAANTGRLQTAIS

General information: TITO was launched using: RESULT: Template: 4Q0S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1080 -7966 -7.38 -36.21 target 2D structure prediction score : 0.33 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -7.38 2D Compatibility (Sec. Struct. Predict.) : 0.33 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.174

(partial model without unconserved sides chains): PDB file : Tito_4Q0S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Q0S-query.scw PDB file : Tito_Scwrl_4Q0S.pdb: