Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKNKTIILGGFTEMKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAALITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDG-FQYFQS-SNESDYIPNIDQALNAGFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVAAAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDKGRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYTLNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTE--GQLSDEAKKAVDEAKEK-IISGDVKVPETPEEN 2HQB Chain:A ((0-286)) -----------------------------------------------------GMVGLLV-EDT----GWNRKAYEGLLNIHSNL------DVDVVLEEGVNSEQKAHRRIKELVDGGVNLIFGHGHAFAEYFSTIHNQYPDVHFVSFNGEVK-GENITSLHFEGYAMGYFGGMVAASMSETHKVGVIAAFPW---QPEVEGFVDGAKYM-N---ES-EAFVRYVGEWTDADKALELFQELQKEQVDVFYPAGDGYHVPVVEAIKDQ------GDFAIGYVGDQA--------DLGGSTILTSTVQHVDDLYVLVAKRFQEGKLESGNL-YYDFQDGVVSLGEFSSVVPDEVREQITDAISTYIQTGQFPH-------

General information: TITO was launched using: RESULT: Template: 2HQB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1621 42845 26.43 154.12 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 26.43 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.559

(partial model without unconserved sides chains): PDB file : Tito_2HQB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HQB-query.scw PDB file : Tito_Scwrl_2HQB.pdb: