Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYN-TEGTITFEAGDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAAADVMNDGSRKPTYVQVTDRRS--TLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVSLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVPGKSVKLAQQYSTKLVWTLEDTPNN--- 5FHY Chain:A ((21-249)) DILKASATQSAVAGTYQIQVNSLATSSKIALQAIADPANAKFNSGTLNISVGDTKL----------PAITVDSSNNTLAGMRDAINQA-GKEAGVSATIITDNSGSRLVLSST-KTGDGKDIKVEVSDDGSGGNTSLSQLAFDPATAPKLSDGA----AAGYVTKAANGEITVDGLKRSIASNSVSDVIDG---VSFDVKAVTEAGKPITLTVSRDDAG-------VKDNVKKFVEAYNTLTKFINEQTVVTKVG

General information: TITO was launched using: RESULT: Template: 5FHY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1028 78984 76.83 363.98 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 76.83 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.149

(partial model without unconserved sides chains): PDB file : Tito_5FHY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FHY-query.scw PDB file : Tito_Scwrl_5FHY.pdb: