Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLTDILVCLSEKQNGRRNNMSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALVGNTFSPNPAMEGQGVIDVL--THIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLIRKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMS------LGISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFI-LAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAIT--TIAPQHLDITQSWVAWG-LGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA 5H7I Chain:C ((15-604)) VPVIDL------NRVSDEEQLLPVVRAILLQHDTFLLKNY---ANKAVLDALLAGLTTKDLPDTSQGFDANFTGTLPLEDDVWLEQYIFDTDPQLRFDRKCRNESLCSIYSRLFKLGLFFAQLCVKSVVSSAELQDCISTSHYATKLTRYFGVLTIFPC-AKAIRYKPSTMATTDNSWVSIDEPDCLLFHTGTLLARW-----SQGMHTTSPLQIDPRANIVSLTIWPSILRLQDAMKFVKELFTVCETVLSLNALSRSTGVPPE---------LHVLLPQISSMMKRKIVQDDILKLLTIWSDAYVVELNSRGE------LTMNLPKRDNLTTLTNKSRTLAFVERAESWYQQVIASSQRQKRYQQFLAMKMTQVFDILFSLTRGQPYTETYLSSLIVDSLQDSTKEASEILAGLQGILPMDISVHQVDGGLKVYRWNSLDKNRFSKLLQIHKSKQQD------------------

General information: TITO was launched using: RESULT: Template: 5H7I.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1754 -142754 -81.39 -342.34 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain C : 0.63 3D Compatibility (PKB) : -81.39 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.093

(partial model without unconserved sides chains): PDB file : Tito_5H7I.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5H7I-query.scw PDB file : Tito_Scwrl_5H7I.pdb: