Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKNKRKQKHMSLLKEGIKMKDTGSSKQITTMTFIGMTCALVASVRNIP---DVA-ATSWTMIFYMLVAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKW-GFVVSWLLWVQMFPGMVMVASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLK-FDMAKIGGKVGVWLGLYVPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEMSSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMEL---NNIAQSISIYCQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQFDVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRYTHAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVILFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN 3GI9 Chain:C ((2-370)) ----------------------ELKNKKLSLWEAVSMAVGVMIGASIFSIFGVGAKIAGRNLPETFILSG-IYALLVAYSYTKLGAKIVSNAGPIAFIHKAIGDNIITGALSILLWMSYVISIALFAKGFAGYFLPLI--API--NTFNIAITEIGIVAFFTALNFFGSKAVGRAEFFIVLVKLLILG-LFIFAGLITI---H--P----SYVIP-DLA-PSAVSGMIFASAIFFLSYMGFGVITNASEHIENPKKNVPRAIFISILIVMFVYVGVAISAIGNLPIDELIKASENALAVAAKPFL---G--NLGFLLISIGALFSISSAMNATIYGGANVAYSLAKDGELPEFFE--RKVWFKST-EGLYITSALGVLFALLF--------NMEGVASITSAVFMVIYLFVILSHYILIDEVG-------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3GI9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1972 -272886 -138.38 -760.13 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain C : 0.67 3D Compatibility (PKB) : -138.38 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.348

(partial model without unconserved sides chains): PDB file : Tito_3GI9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3GI9-query.scw PDB file : Tito_Scwrl_3GI9.pdb: