Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPE-EFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPG-E----ITDNNGILTFNFSDPEGNYFVIGKAESSD 5UHJ Chain:A ((3-128)) ----------------AMISHIGTIDVFVNDQDKAIDFYVNTLGFELREDQAFGPMRWVEVAPAGS-QTRIVLCTKHFPVY-EEGKIGRFTDIQLVTEDIKATHEELVRRGVNFTRAPEQMP--ANASFQDPDGNEFLLLQPS---

General information: TITO was launched using: RESULT: Template: 5UHJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 452 -6685 -14.79 -57.13 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -14.79 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.396

(partial model without unconserved sides chains): PDB file : Tito_5UHJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5UHJ-query.scw PDB file : Tito_Scwrl_5UHJ.pdb: