Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQMNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGFNLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEK-KVVLA------GGKKLDFDRLILATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIM--NAKKIAIIGAGQAGIEIAEGLASRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVETEKRKENYDLVLLANHSRPDNQMWEE-QLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVTFRPTNEKMYVSLVNNAVRTARNVSKTISGSQT-KDQGTYRPVGNQWFGYFLGSVGLTEEESIFYPQKIETGYVDYK--LTAIN-QSKVKLKFLFDSE-KKILGAQLQSKKEMFHLLDQLTIAVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED 4EQS Chain:B ((1-424)) --PKIVVVGAVAGGATCASQIRRLDKESDIIIFEKDRDMSFANCALPYVIGEVVEDRRYALAYTPEKFYDRKQITVKTYHEVIAINDERQTVSVLNRKTNEQFEESYDKLILSPGASANSLGFESDI--TFTLRNLEDTDAIDQFIKANQVDKVLVVGAGYVSLEVLENLYERGLHPTLIHRSDKINKL-MDADMNQPILDELDKREIPYRLNEEINAINGN--EITFKSGKVEHYDMIIEGVGTHPNSKFIESSNIKLDRKGFIPVNDKFETNVPNIYAIGDIATSHYRHVDLPASVPLAWGAHRAASIVAEQIAGNDTIEFKGFLGNNIVKFFDYTFASVGVKPNELKQFD--YKMVEVTQGAHANYYPGNSPLHLRVYYDTSNRQILRAAAVGKEGADKRIDVLSMAMMNQLTVDELTEFEVAFAPPYSH---------------

General information: TITO was launched using: RESULT: Template: 4EQS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2340 5554 2.37 13.58 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain B : 0.74 3D Compatibility (PKB) : 2.37 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.434

(partial model without unconserved sides chains): PDB file : Tito_4EQS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EQS-query.scw PDB file : Tito_Scwrl_4EQS.pdb: