Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTRRKPMKFMTLNTHSWLEP-----EPEKKLQELADKILLEDYEVIALQEINQLLES-EEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSH-----------IGYDKYEEGNALLTKKTLES-QVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDK------KSGFYFEWKNLENYFLE----TRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKAATVEKKI---DGWE-QNTEKLRIDFIFVPEGMQ-VKKYQRI------------F-DGIDSPIISDHYGVEIELDVNE 3WCX Chain:A ((7-298)) ----TPSLKVLTYNTFLMSTGLYPNWGQEHRAREIAAAGFFQGNDVVVLQEAFDNAAADGLK---------------------------AAAAD-RYPYQTPVVGRSRDGWDATGGKYSATTPEDGGVTVLSKWPIVRKEQVIFNDACGADWWSNKGFAYVVLNVGGTRVHVVGTHAQSTDSGCAAGEAAADRSRQFRQIDAFLDAKNIPADEQVMLAGDLNVDSHSAEYASMLAD-GDLAPADSRAGH---PYSFDTKENSIAAYRYPTDPREDLDYVLHRNGHARPAGWRNTVVQETSAPWTVSSWKRYTYTDLSGHYPVIAGAN---

General information: TITO was launched using: RESULT: Template: 3WCX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1375 59162 43.03 241.48 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 43.03 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.291

(partial model without unconserved sides chains): PDB file : Tito_3WCX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3WCX-query.scw PDB file : Tito_Scwrl_3WCX.pdb: