Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MINMEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTTFSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQINASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL 5WTQ Chain:A ((14-123)) ---DVSLHNFSARLWEQLVHFHVMRLTDSLFLWVGA-T-PHLRNLAVAMSSRYDSI--PVSTSLLGDTSDTTSTGLAQRLARKTNKQVFVSYNLQNTDSN------FALLVE---NRIKEEMEAFP----------------

General information: TITO was launched using: RESULT: Template: 5WTQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 383 -30370 -79.29 -276.09 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -79.29 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.235

(partial model without unconserved sides chains): PDB file : Tito_5WTQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5WTQ-query.scw PDB file : Tito_Scwrl_5WTQ.pdb: