Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------------------------------------------------------------------------MNGFMDKLSEKIMPLANLLGQ--NRYLTVLRDAFMLSFP----LTMFGSIVVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGAVSFASFLILTPFAM----------KTAGGEDVTGVLSLDRLGAKGMFIGMIAA---FLAGEIYCRITKRGWQ-------IKMPDGVPPAV---------TKPFAALIPAVVTLTIFLVINAVMTGIFN---------ANLHDVVYEVIQKPLTGLGSSLPATLIALF-----FVQFLWFFGLHGQI---IVNSVMDPIWNTLMLDNLEAYQHGKELPHIITKPFMETFT----------------VGIGGSGMTLAVV--LLMAFVLKKKQYRDVGRLALAP--------GIFNVNEPAIFGLPIVLNA---TILIPWVVAPLIV-----------TTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLGGMLQV------IDFVIV----------AFIWYPFLKVLDKQPDLDIA----- 4RIC Chain:A ((1-631)) TGHPYYLRSFLVVLKTVLENEDDMLLFDEQEKGIVTKFYQLSATGQKLYVRLFQRKLSWIKMTKLEYEEIALDLTPVIEELTNAGFLQTESE-LQELSEVLELLSAPELKSLAKTFHLVNPNGQKQQLVDAFLKLAKQRSVIGAVILKRAKALAGQSVRICKGPRAVFSRILLLFSLTDSMEDEDAACGGQGQLSTVLLVNLGRMEFPSYTINRKTHIFQDRDDLIRYAAATHMLSDISSAMANGNWEEAKELAQCAKRDWNRLKNHPSLRCHEDLPLFLRCFTVGWIYTRILSRFVEILQRLHMYEEAVRELESLLSQRIYCPDSRGRWWDRLALNLHQHLKRLEPTIKCITEGLADPEVRTGHRLSLYQRAVRLRESPSCKKFKHLFQQLPEMAVQDVKHVTITGRLCKSVFVMVLCSVEELALAHYRRSGFDQGIHGEGSTFSTLYGLLLWDIIFMDGIPDVFRNACQAFPLDLCTDSFFTSRRPALEARLQLIHDAPEESLRAWVAATWHEQEGRVASLVSWDRFTSLQQAQDLVSCLGGPVLSGVCRHLAADFRHCRGGLPDLVVWNSQSRHFKLVEVKGPNDRLSHKQMIWLAELQKLG--AEVEVCHVVAV

General information: TITO was launched using: RESULT: Template: 4RIC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1687 -87519 -51.88 -208.38 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -51.88 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.079

(partial model without unconserved sides chains): PDB file : Tito_4RIC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RIC-query.scw PDB file : Tito_Scwrl_4RIC.pdb: