Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAESTTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEYAPKAVENFMTHAKDGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNI-RGALSMARSQDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGMDVVDAIANIETD-SSDKPKEEVKIDKINILQEAKQK 1ZKC Chain:B ((15-174)) ----------------------------------------------------------------VPRGSGYVRLHTNKGDLNLELHCDLTPKTCENFIRLCKKHYYDGTIFHRSIRNFVIQGGDPTGTGTGGESYWGKPFKDEFRPNLSHTGRGILSMANS-GPNSNRSQFFI----------------------TFRSCAY--LDKKHTIFGRVVGGFDVLTAMENVESDPKTDRPKEEIRIDATTVF------

General information: TITO was launched using: RESULT: Template: 1ZKC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 824 -12824 -15.56 -81.16 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : -15.56 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.472

(partial model without unconserved sides chains): PDB file : Tito_1ZKC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ZKC-query.scw PDB file : Tito_Scwrl_1ZKC.pdb: