Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDVRKSLQTILLKEAISMKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKVTASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSARLDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDTLLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQLGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANGNVTLKTWD-SNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNK----RVRADLVFYLPKRVYDHAAIKLLNGNIMIE-TLEAKDIYTKST-NGNIIVDQ-LTATMLEVEG-VNGNIDIRNGNILDSIIET-VNGTVTFG-ATAENLSVSLV-NGDVRLTIKEDNLKKVEAS-SVNGNVKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQLSTTTGSIYLKDTDK 4OPW Chain:B ((17-223)) --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TIDDFNAIKFDG-VIDFNYEQSESTPHIEITVDEN------------LHPYVNIDIQDRVLTVGFKGAKVDHFTKFIVKTNSKW--LKEVKAS-GNANFIANSPLKGDELKINANSNCLVQLKQKVEVGKLDLNVSGSANMVVNELKTDKLECSINGSGTINLKAGNAEEADYSITTDGEIMAFGVA--VPEVNCKITGKGSAQIHPTDN--LKATI-VGKGNIRYKG----------------------------------------------

General information: TITO was launched using: RESULT: Template: 4OPW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1146 47842 41.75 246.61 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain B : 0.65 3D Compatibility (PKB) : 41.75 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.448

(partial model without unconserved sides chains): PDB file : Tito_4OPW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4OPW-query.scw PDB file : Tito_Scwrl_4OPW.pdb: