Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------MAWFTLILFGLMTVATLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIG-ILLSAIFFLLNIGTFTKNWRMTLVGIYFYFYPFGVMALSEVMHWD-----NHWFIAGSLLLGLILL----HVMLTKLYQHYGIGRTIFWYVFSIPFALLTALLPSRLKTKIKG-YHKIK 3IFU Chain:A ((6-180)) DRCTCTPNARVFVAEGQVYCTRCLSARSLLPLNLQVPELGVLGLFYRPEEPLRWTLPRAFPTVECSPAGACWLSAIFPIARMTSGNLNFQQRMVRVAAEIYRAGQLTPTVLKTLQVYERGCRWYPIVGPVPGVGVYANSLHVSDKPFP--------------GATHVLTNLPLPQRPKPEDFCPFECAM

General information: TITO was launched using: RESULT: Template: 3IFU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 646 -54747 -84.75 -360.17 target 2D structure prediction score : 0.27 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -84.75 2D Compatibility (Sec. Struct. Predict.) : 0.27 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.059

(partial model without unconserved sides chains): PDB file : Tito_3IFU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3IFU-query.scw PDB file : Tito_Scwrl_3IFU.pdb: