Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQRDLEKANITIL-PFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFIQNYTSIAVTGSHGKTSTTGLLSHVLS--GVRPTSYLIGDGTG-H--G---DPQAEFFSFEACEYRRHFLAYSPDYAIMTNIDFDHPDYYT-SIDDVFTAFQTMAGQVK--KAIFAYGDDAYLRKLKANV--PIYYYGVTE-NDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVIAVAY-FEKLDLKEVAEEMLTFPGVKRRFSEKI-----VADMTVVDDYAHHPAEIKATIDGARQKYPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQG-NVKIEDLGAKIKKGG----EVI-KENNVSPLL---DYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL 1GQY Chain:B ((14-474)) EMRRVQQIHFIGIGGAGMSGIAEILLNEGYQISGSDIAD-GVVTQRLAQAGAKIYIGHAEEHIEGASVVVVSSAIKDDNPELVTSKQKRIPVIQRAQMLAEIMRFRHGIAVAGTHGKTTTTAMISMIYTQAKLDP-TFVNGGLVKSAGKNAHLGA-SRYLIAEADESDASFLHLQPMVSVVTNMEPDHMDTYEGDFEKMKATYVKFLHNLPFYGLAVMCADDPVLMELVPKVGRQVITYGFSEQADY-RIEDYEQTGFQGHYTVICPNNERINVLLNVPGKHNALNATAALAVAKEEG-IANEAILEALADFQGAGRRFDQLGEFIRPNGKVRLVDDYGHHPTEVGVTIKAAREGWGDKRIVMIFQPHRYSRTRDLFDDFVQVLSQVDALIMLDVYA-AGEAPIVGADSKSLCRSIRNLGKVDPILVSDTSQLGDVLDQIIQDGDLILAQGAGSVSKISRGLAESWK-------

General information: TITO was launched using: RESULT: Template: 1GQY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2388 -9932 -4.16 -23.04 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : -4.16 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.524

(partial model without unconserved sides chains): PDB file : Tito_1GQY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GQY-query.scw PDB file : Tito_Scwrl_1GQY.pdb: