Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNVAPLYAEKI--MFQLRQANFEPVLSIV----PAGETSKSLEQAAELYDFLADND--FTRSDGILALGGGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPAGVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNE--------LKDEKDLLEHAV--------PIITATLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHAEKIGLSPTGSTAALERILQKFHLPITYDLSH-----------PDELFHAITHDKKARGSQLNIILLEKIGQAKIVPVPTETFKDYLGGL 1SG6 Chain:B ((41-372)) -------------------------------------LVTDTNIGSIYTPSFEEAFRKRAAEITPSPRLLIYNRPPGEVSKSRQTKADIEDWMLSQNPPCGRDTVVIALGGGVIGDLTGFVASTYMRGVRYVQVPTTLLAMVDSSIGGKTAIDTPLGKNLIGAIWQPTKIYIDLEFLETLPVREFINGMAEVIKTAAISSEEEFTALEENAETILKAVRREVTPGEHRFEGTEEILKARILASARHKAYVVSA----GGLRNLLNWGHSIGHAIEAILTPQIL-HGECVAIGMVKEAELARHLGILKGVAVSRIVKCLAAYGLPTSLKDARIRKLTAGKHCSVDQLMFNMAL--------KKIVLLSAIG-------------------

General information: TITO was launched using: RESULT: Template: 1SG6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1364 -22845 -16.75 -80.16 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : -16.75 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.527

(partial model without unconserved sides chains): PDB file : Tito_1SG6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1SG6-query.scw PDB file : Tito_Scwrl_1SG6.pdb: