Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKMSDEALKIIEESQNREKLKQVIDLIEERLVE-HAIVPTELQWTILINHLNEMLKRSQRKETIPVVDRDLFKEVS---KEAITISDEIVR----SIG-NLTDDEIYVLSIHFETAKNN 3RIO Chain:A ((83-179)) ------------------KLVDISNDVIYHISNRTNHSLNEHIHIALTDHIAFAIKRQQQGFDMKN---PFLMETQSLYPEEYQIAKEVIDMINEKAGLCLPEGEIGFIALHIHSALT-

General information: TITO was launched using: RESULT: Template: 3RIO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 264 -5255 -19.90 -59.71 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -19.90 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.665

(partial model without unconserved sides chains): PDB file : Tito_3RIO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3RIO-query.scw PDB file : Tito_Scwrl_3RIO.pdb: