Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETALGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKDLNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQREDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK 2OO7 Chain:B ((1-212)) MNLVLMGLPGAGKGTQGERIVEDYGIPHISTGDMFRAAMKEETPLGLEAKSYIDKGELVPDEVTIGIVKERLGKDDCERGFLLDGFPRTVAQAEALEEILEEYGKPIDYVINIEVDKDVLMERLTGRRICSVCGTTYHLVFNPPKTPGICDKDGG-ELYQRADDNEETVSKRLEVNMKQIQPLLDFYSEKGYLANVNGQRDIQDVYADVKDLL--

General information: TITO was launched using: RESULT: Template: 2OO7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1003 -91207 -90.93 -430.22 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.92 3D Compatibility (PKB) : -90.93 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.92 QMean score : 0.591

(partial model without unconserved sides chains): PDB file : Tito_2OO7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2OO7-query.scw PDB file : Tito_Scwrl_2OO7.pdb: