Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQERLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGK-LPEVMEVSQD-FAKVMDKDELIDQTAVQNVIE-EAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKLSEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLDDEGEVTIDTEKMREALSFYQNLSQYTM-PGS--NDVTEIKDAFMNGTAPMAIYSTYILPSVYEAG---NSENIGFA-IPTQKDQA--VYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKWVLMSPGGAQPVNKQVVELDGYKE-NEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKMGDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK 5IAI Chain:A ((24-419)) ----------------------KP----LDGVTLTLASQND--PFGAVLTKLAAEFKQD-TGADLKVEVMDYGTLLTKTTADFVGKTKGYDLVTMDIVWAGAYQANGYSVDLTDWVKRDAAELDLDDIYPVILQSLGQYKGHYV-AFPFAAYANVLAYRKDLFQAAGLPVPTTVEELVSDAKKLTDPSKKQYGFVANGQKGPAVAQDWMQYNNQMGGSILDNDGKPALNSPENVKSLTVYKQLFVETAPPGAIEYDWGGREESFRQGAAAMMQTWSVGAPGYSDPASSNVVGKVGITTAPVGKGVPPQYGVGGWGMAINADIDPKQKEAAWTFIKWLVSKKIHKEFNMD--GAGSFMRKSQMTDPDLTAKFDFLPVVAK-------TYENGNGEYRPRIPEYPEI-----QDILGSAVNSVLAGAAEPQAALDEAQVEAK

General information: TITO was launched using: RESULT: Template: 5IAI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2207 100051 45.33 261.23 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 45.33 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.436

(partial model without unconserved sides chains): PDB file : Tito_5IAI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5IAI-query.scw PDB file : Tito_Scwrl_5IAI.pdb: