Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREMPFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSAREGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKA---------------ARIKSVI-----GEDPLLEFGTRRAQEL---DAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYG--------NDYEAFMAYAKTH--KDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVREQLDAAGFT--EAKIYASNDLDEATILNLKM--QKAKIDVWGVGTKLITAY-DQPALGAVFKLVSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEEAIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEEYKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA 1YIR Chain:D ((10-395)) ----ERIVQNLLDTDFYKLTMMQAVLHNYP-NAEVEWEFRCRNQEDL--RLYLPAIREQLEYLAGLAISDEQLAFLERIPFLAPDFIRFLGLFRFNP--RYVQTG--IENDEFFLRLKGPWLHVILFEVPLLAMISEVRNRARYPAATVEQARERLQEKFDWLRREASAEELAGFKMADFGTRRRFSYRVHEAVVSGLKEDFPGCFVGTSNVHLARKLDLKPLGTMAHEWLMAHQQLGPRLIDSQSAALDCWVREYRGLLGIALTDCITTDA-FLRDFDLYFA-----KLFDGLRHDSGDPLLWAEKTIAHYLKLGIDPLTKTLVFSDGLDLPRALKIYRALQGRINVSFGIGTHFTCDLPGVEPMNIVVKMSACNG-----HPVAKISDTP-------PDFIHYLKH------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1YIR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1709 -93423 -54.67 -273.97 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain D : 0.65 3D Compatibility (PKB) : -54.67 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.434

(partial model without unconserved sides chains): PDB file : Tito_1YIR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1YIR-query.scw PDB file : Tito_Scwrl_1YIR.pdb: