Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MTHKIGVVGDKDSVLPFKLFG-------------FDVRYG-TTKQTVRQAIEEMAKN-EYGVIYV-TEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNIL 4IX9 Chain:A ((3-94)) EKRTLIAVIADEDTTTGLLLAGIGQITPETQEKNFFVYQEGKTTKEEITDKFNHFTEERDDIAILLMNQHIAENIRARVDSFTNA-FPAILEI---------------------------

General information: TITO was launched using: RESULT: Template: 4IX9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 284 7370 25.95 98.26 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : 25.95 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.584

(partial model without unconserved sides chains): PDB file : Tito_4IX9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4IX9-query.scw PDB file : Tito_Scwrl_4IX9.pdb: