Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIGLIG-SGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTELPELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFSFGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYNWQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADWLKDQSPGYYKLEDLLMDKGV 1VM6 Chain:C ((13-225)) MKYGIVGYSGRMGQEIQKVFSEKGHELVL----KVDVNGVEELDSPDVVIDFSSPEALPKTVDLCKKYRAGLVLGTTALKEEHLQMLRELSKEVPVVQAYNFSIGINVLKRFLSELVKVLEDWDVEIVETHHRFKKDAPSGTAILLESAL-------------GK-----SVPIHSLRVGGVPGDHVVVFGNIGETIEIKHRAISRTVFAIGALKAAEFLVGKDPGMYSFEEVIF----

General information: TITO was launched using: RESULT: Template: 1VM6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1084 4275 3.94 20.17 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain C : 0.83 3D Compatibility (PKB) : 3.94 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.511

(partial model without unconserved sides chains): PDB file : Tito_1VM6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VM6-query.scw PDB file : Tito_Scwrl_1VM6.pdb: