Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------VYHGKISRETGEKLLLATG-LDGSYLLRDSESVPGVYCLCVLYHGYIYTYRVSQTETGSWSAETAPGVHKRYFRKIKNLISAF--------------------- 1CSZ Chain:A ((1-112)) GSRRASVGSHEKMPWFHGKISREESEQIVLIGSKTNGKFLIRARDNN-GSYALCLLHEGKVLHYRIDKDKTGKLSIPE----G-KKFDTLWQLVEHYSYKADGLLRVLTVPCQKIGTQ

General information: TITO was launched using: RESULT: Template: 1CSZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 239 1028 4.30 13.53 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : 4.30 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.405

(partial model without unconserved sides chains): PDB file : Tito_1CSZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1CSZ-query.scw PDB file : Tito_Scwrl_1CSZ.pdb: