Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence NEDCIVSLVP-----DGGSFARKTLRSSPGQQHDLQWGKYAFTIRVENGCAGKKTAGTIPPAHFVSVGRAAS-----LKPTSILRDGDPRPPT-AVYPPRQPPRTEGRRTRYGSSGG--AVTVGAR 1AKP Chain:? ((1-114)) -ASAAVSVSPATGLADGATVTVSASGFATSTSATA--LQCAI---LADGRGACNVA----EFHDFSLSGGEGTTSVVVRRSFTGYVMPDGPEVGAVDCDTAPGGCEIVVGGNTGEYGNAAISFG--

General information: TITO was launched using: RESULT: Template: 1AKP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 335 -991 -2.96 -9.81 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -2.96 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.166

(partial model without unconserved sides chains): PDB file : Tito_1AKP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1AKP-query.scw PDB file : Tito_Scwrl_1AKP.pdb: