Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------------------------------FVQCNHHLLYNGRHWGTIRKKAGWAVRFYEEKPGQPKRLVAICKNASPVHCNYLKCTN-------LAAGFSAGTSTDVLSSGTVGSIGNDPQAQRQ---------------------------------------------------------------------------------------------------------------------------------------------- 2JFV Chain:A ((19-288)) MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARCPYGPRPAEQVIQYTWEMTDYLVEQ--GIKMLVIACNTATAVALEEIKAALSIPVIGVILPGTRAAVKKT--QNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEYHSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGEVSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIEL

General information: TITO was launched using: RESULT: Template: 2JFV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 330 -4091 -12.40 -48.12 target 2D structure prediction score : 0.36 Monomeric hydrophicity matching model chain A : 0.53 3D Compatibility (PKB) : -12.40 2D Compatibility (Sec. Struct. Predict.) : 0.36 1D Compatibility (Hydrophobicity) : 0.53 QMean score : 0.060

(partial model without unconserved sides chains): PDB file : Tito_2JFV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2JFV-query.scw PDB file : Tito_Scwrl_2JFV.pdb: