Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------------------------------------------------------------------------------------------------------MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMFHCEGCYNV-ATWSFNAGIPYTAELEEQIMAD-LAQPYVQGLTLLGGEPFL-NTGILLPLVKRIRKELPD-KDIWSWTGYTWEEMMLETPDKLELLSLIDILVDGRYDRTKR-NLMLQFRGSSNQRIIDVQ-KSLKS-GQVVIWDKLNDGKESYEQVKRE-------- 4DVG Chain:B ((8-312)) EVVQQKFAIVAKEMKIDNPELITIPNQWKLVQEYEKKQKKDIRIQLNAQKTGNWRNAITDPKYLADLLKTRDDMDLLNEMVVVFRSSSVSFIKTFVSVGGLANLMAIYKKKIEAENSNTAIDEERKCCEVLRYV---FAEEDATVALIEIDG---GVELLLKGMNSKRITPDNQLDILLEITLTSSMVEHPSQEGL-YLGGDVCVMN------AFSNLVSEGVDMKKFLSFFSLFSKSK--SEKFKHASLVLINNLID-QPELEHRMDVRNSFIEIGLVNELE-NMKNTEWMKIDKIKDSINDFFDSWEEDKKEVESRFDDL

General information: TITO was launched using: RESULT: Template: 4DVG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 650 24863 38.25 138.90 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain B : 0.59 3D Compatibility (PKB) : 38.25 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.245

(partial model without unconserved sides chains): PDB file : Tito_4DVG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4DVG-query.scw PDB file : Tito_Scwrl_4DVG.pdb: